Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle
Identifieur interne : 003B54 ( Main/Exploration ); précédent : 003B53; suivant : 003B55Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle
Auteurs : Gui-Mei Li [Japon] ; Yong-Gang Li [Japon, Thaïlande] ; Masanobu Yamate [Japon] ; Shu-Ming Li [Japon] ; Kazuyoshi Ikuta [Japon, Thaïlande]Source :
- Microbes and infection [ 1286-4579 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Animaux, Cellules Vero, Cholestérol (déficit), Cholestérol (métabolisme), Cholestérol (pharmacologie), Cyclodextrines bêta (pharmacologie), Cytométrie en flux, Microdomaines membranaires (métabolisme), Microdomaines membranaires (virologie), Peptidyl-Dipeptidase A (métabolisme), Réplication virale (), Réplication virale (physiologie), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (croissance et développement), Virus du SRAS (génétique), Virus du SRAS (physiologie), Étapes du cycle de vie.
- MESH :
- croissance et développement : Virus du SRAS.
- déficit : Cholestérol.
- génétique : ARN messager, Virus du SRAS.
- métabolisme : ARN messager, Cholestérol, Microdomaines membranaires, Peptidyl-Dipeptidase A, Syndrome respiratoire aigu sévère.
- pharmacologie : Cholestérol, Cyclodextrines bêta.
- physiologie : Réplication virale, Virus du SRAS.
- virologie : Microdomaines membranaires, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Cholesterol (deficiency), Cholesterol (metabolism), Cholesterol (pharmacology), Coronavirus, Early stage, Flow Cytometry, Life Cycle Stages, Life cycle, Lipid rafts, Membrane Microdomains (metabolism), Membrane Microdomains (virology), Peptidyl-Dipeptidase A (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), SARS Virus (genetics), SARS Virus (growth & development), SARS Virus (physiology), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Vero Cells, Virus Replication (drug effects), Virus Replication (physiology), beta-Cyclodextrins (pharmacology).
- MESH :
- chemical , deficiency : Cholesterol.
- chemical , genetics : RNA, Messenger.
- chemical , metabolism : Cholesterol, Peptidyl-Dipeptidase A, RNA, Messenger.
- chemical , pharmacology : Cholesterol, beta-Cyclodextrins.
- drug effects : Virus Replication.
- genetics : SARS Virus.
- growth & development : SARS Virus.
- metabolism : Membrane Microdomains, Severe Acute Respiratory Syndrome.
- physiology : SARS Virus, Virus Replication.
- virology : Membrane Microdomains, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Flow Cytometry, Life Cycle Stages, Vero Cells.
Abstract
Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.
Affiliations:
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aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University</s1><s2>Nonthaburi 11000</s2><s3>THA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Thaïlande</country><wicri:noRegion>Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University</wicri:noRegion></affiliation></author><author><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Li, Shu Ming" sort="Li, Shu Ming" uniqKey="Li S" first="Shu-Ming" last="Li">Shu-Ming Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University</s1><s2>Nonthaburi 11000</s2><s3>THA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Thaïlande</country><wicri:noRegion>Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Microbes and infection</title><title level="j" type="abbreviated">Microbes infect.</title><idno type="ISSN">1286-4579</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Microbes and infection</title><title level="j" type="abbreviated">Microbes infect.</title><idno type="ISSN">1286-4579</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cholesterol (deficiency)</term><term>Cholesterol (metabolism)</term><term>Cholesterol (pharmacology)</term><term>Coronavirus</term><term>Early stage</term><term>Flow Cytometry</term><term>Life Cycle Stages</term><term>Life cycle</term><term>Lipid rafts</term><term>Membrane Microdomains (metabolism)</term><term>Membrane Microdomains (virology)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Vero Cells</term><term>Virus Replication (drug effects)</term><term>Virus Replication (physiology)</term><term>beta-Cyclodextrins (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Animaux</term><term>Cellules Vero</term><term>Cholestérol (déficit)</term><term>Cholestérol (métabolisme)</term><term>Cholestérol (pharmacologie)</term><term>Cyclodextrines bêta (pharmacologie)</term><term>Cytométrie en flux</term><term>Microdomaines membranaires (métabolisme)</term><term>Microdomaines membranaires (virologie)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Réplication virale ()</term><term>Réplication virale (physiologie)</term><term>Syndrome respiratoire aigu sévère (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term><term>Étapes du cycle de vie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Cholesterol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cholesterol</term><term>Peptidyl-Dipeptidase A</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cholesterol</term><term>beta-Cyclodextrins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Cholestérol</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Membrane Microdomains</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Cholestérol</term><term>Microdomaines membranaires</term><term>Peptidyl-Dipeptidase A</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Cholestérol</term><term>Cyclodextrines bêta</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réplication virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Microdomaines membranaires</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Membrane Microdomains</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Flow Cytometry</term><term>Life Cycle Stages</term><term>Vero Cells</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus</term><term>Cytométrie en flux</term><term>Réplication virale</term><term>Stade précoce</term><term>Cycle développement</term><term>Syndrome respiratoire aigu sévère</term><term>Microdomaine de type raft</term><term>Étapes du cycle de vie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.</div></front></TEI><affiliations><list><country><li>Japon</li><li>Thaïlande</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Li, Gui Mei" sort="Li, Gui Mei" uniqKey="Li G" first="Gui-Mei" last="Li">Gui-Mei Li</name></noRegion><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><name sortKey="Li, Shu Ming" sort="Li, Shu Ming" uniqKey="Li S" first="Shu-Ming" last="Li">Shu-Ming Li</name><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name></country><country name="Thaïlande"><noRegion><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name></noRegion><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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